International Journal of Scientific Research and Engineering Development

Petroleum contamination poses significant ecological threats due to its persistence and toxicity. This study aimed to isolate, screen, and optimize indigenous hydrocarbon-degrading bacteria from petroleum-impacted environments and assess their degradation capabilities under varying environmental conditions. Fifteen isolates were obtained via enrichment culture, and three strains—Bacillus subtilis, Pseudomonas putida, and Bacillus cereus—were selected for further characterization. Optimal degradation conditions were determined across a range of temperature, pH, salinity, hydrocarbon type, and concentration. Quantitative degradation analysis was performed using gravimetric methods and GC-MS profiling. Pseudomonas putida (B3 isolate) exhibited the highest degradation (85%) under optimal conditions (30°C, pH 7.0, 2% NaCl, 1% crude oil). GC-MS confirmed the breakdown of major hydrocarbons into less toxic intermediates. These findings underscore the potential of indigenous bacterial strains in sustainable bioremediation and highlight the importance of environmental optimization for enhanced biodegradation performance.

Moumita Roy, Dr.Madhurima De Roy,"Optimizing Conditions and Evaluating Hydrocarbon Degradation Efficiency of Indigenous Bacterial Isolates from Petroleum-Contaminated Sites" International Journal of Scientific Research and Engineering Development, V9(1): Page(1921-1926) Jan-Feb 2026. ISSN: 2581-7175. www.ijsred.com. Published by Scientific and Academic Research Publishing.