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Immobilization of Alkaline Metalloprotease for Stain Removal From Fabric Using Magnetic Nanoparticles

	International Journal of Scientific Research and Engineering Development (IJSRED)	Full Text:
	Published Issue : Volume-3 Issue-6
	Year of Publication : 2020
	Unique Identification Number : IJSRED-V3I6P1
	Authors : Sachin, Mr. Brajesh Singh
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Abstract :

Protease enzymes play an important role invarious type of industries such as food, pharmaceutical, cosmetic ,detergent , leather , meat and textile industry etc. According to recent studies protease immobilization onto magnetic nanoparticles is effective in fabric cleaning. Magnetic nanoparticles increase the effectiveness of enzyme immobilization and its stability. Further there is an increment in enzyme reusability as well as improvement in enzyme recovery because of their low toxicity factor. Although, implementation of these nanomaterial are restricted due to the efficacy of their recovery processes. With the help of an external magnet these magnetic nanoparticles can be easily removed from the reaction mixture, therefore it is recommended to use solution of these nanoparticles. Immobilized enzyme compare to free enzyme in solution are more vigorous and more resistant to environmental changes. Diversity of an immobilized system allows an easy recovery of both enzyme and product. Nanomaterials for enzymes immobilization consists of novel and interesting matrices as they have an advantage of having high surface to volume ratio. The behavior of enzymes immobilized on these matrices is due to their Brownian motion effect.